These features indicate the potential regulatory landscape supporting the assignment of putative cell types and reveal repetitive usage of regulators in excitatory and inhibitory cells for determining subtypes. We constructed taxonomies of these epigenetic types, annotated with signature genes, regulatory elements and transcription factors. We identified 161 cell clusters with distinct spatial locations and projection targets. Here we carry out a comprehensive assessment of the epigenomes of mouse brain cell types by applying single-nucleus DNA methylation sequencing 1, 2 to profile 103,982 nuclei (including 95,815 neurons and 8,167 non-neuronal cells) from 45 regions of the mouse cortex, hippocampus, striatum, pallidum and olfactory areas. Mammalian brain cells show remarkable diversity in gene expression, anatomy and function, yet the regulatory DNA landscape underlying this extensive heterogeneity is poorly understood. Nature volume 598, pages 120–128 ( 2021) Cite this article DNA methylation atlas of the mouse brain at single-cell resolution
0 kommentar(er)
